Views Read Edit View history. Among the six plant extracts, P. retrofractum had the most potent extract with an LD50 value of 5.575 µg/larva. q�I�����[�]BE�㇟�I���ڕfcߛU}���n�s��l� This noctuid is often found as part of a complex of lepidopteran and non-lepidopteran foliar feeders but may also injure tubers and roots. On factors causing outbreak of Spodoptera litura (Fabricius). Aktar MW, Sengupta D, Chowdhury A. J Trop Life Sci. The authors are grateful and would like to thank the Science Achievement Scholarship of Thailand (SAST) and the funding from the Department of Zoology, Faculty of Science, Kasetsart University. Awoyinka OA, Oyewole IO, Amos BMW, Onasoga OF. Identification of insecticidal constituents of the essential oil of Acorus calamus rhizomes against Liposcelis bostrychophila badonnel. The no-choice bioassay investigated the antifeedant effect. Senthil-Nathan S. Physiological and biochemical effect of neem and other Meliaceae plants secondary metabolites against lepidopteran insects. They are biodegradable and ecologically safe and an important component of IPM programs [6]. VB mainly thanks the research funding support from ARDA, Thailand, and RSA58 from the Thailand Research Funding. Upadhyay and Jaiswal found that 0.2 μl of P. nigrum oil significantly repelled Tribolium castaneum [18]. Asian J Plant Sci. An observed mortality less than expected suggested an antagonistic effect of the mixtures. Among all extracts, P. retrofractum was the most effective extract against S. litura, followed by A. calamus and P. nigrum; whereas, A. galangal, S. trilobata, and C. longa were less efficient (Table 2). 13C NMR (100 MHz, CDCl3): δ 165.59, 148.34, 148.26, 142.64, 138.38, 131.12, 125.51, 122.65, 120.20, 108.64, 105.82, 101.42, 47.07, 43.38, 26.87, 25.80, 24.81. endstream endobj 93 0 obj <> endobj 94 0 obj <> endobj 95 0 obj <>stream We also determined absolute P. This acts as a protective layer from parasites predating on eggs. Zhou BG, Wang S, Dou TT, Liu S, Li MY, Hua RM, et al. Ind Crops Prod. Moreover, significant increases between the control and treated groups (p < 0.05, df = 7) in vivo were noted for GST (1.488 µg/larvae P. retrofractum + 6.735 µg/larvae A. calamus and 1.488 µg/larvae P. retrofractum + 3.294 µg/larvae A. calamus) (Table 6). extracts on the insect pest, Plutella xylostella (Linnaeus). The extraction method was modified from Feyereisen [24], and surviving larvae were placed in a microtube and kept on ice. These results support a role for ABA increasing the tolerance of honeybee larvae to low temperatures through priming effects. 2001;49:715–20. Schmidt and Streloke [42] investigated the chemical composition of A. calamus rhizome using beta-asarone as the major compound for control Prostephanus truncatus (Horn). Article  Alpinia galanga, P. nigrum and P. retrofractum extracts obtained were dark brown gum, whereas S. trilobata and C. longa extracts were dark green gum and orange gum, respectively. 2013;4:1–17. Spodoptera litura (Lepidoptera: Noctuidae) is a polyphagous insect pest that feeds on at least 87 plant species in over 40 plant families, including many vegetables, fruit, cotton, groundnut, chili, tobacco, castor, lady finger, cauliflower, and pulses, in many Asian countries, such as Thailand, China, Japan, India [1,2,3]. After treatment, larvae were maintained in the insect-rearing room and allowed to feed on an artificial diet. The median lethal dose (LD50) at 24 h after exposure was calculated by Probit analysis using the Statplus program (version 2017, Analyst Company, Canada). In total, larvae belonging to 58 fish families were studied. Therefore, the ratio and concentration of the mixed compounds are essential given that a suitable concentration could possibly affect detoxification enzymes. Springer Nature. Ganjewala D, Srivastava AK. J Sci Technol. Its compound has numerous activities against insects, such as antifeedant, growth inhibition, growth regulation, reduced fecundity and sterility, and inhibition of protein synthesis, as well as toxic effects in a wide variety of insect taxa, including Lepidoptera [7, 8]. Enzyme solution (40 µl) was mixed with p-nitrophenylacetate (pNPA) (10 mM in DMSO) and potassium phosphate buffer (50 mM, pH 7.4). The other crude extract that showed the highest S. litura control in our results is A. calamus. Botanical extract products have become more prominent in assessments of current and future pest control alternatives. Comparative growth inhibitory and antifeedant effets of plant extracts and pure allelochemicals on four phytophagous insect species. 2004;16:332–5. 2014;2:86–92. 2009;2:1–12. This compound also showed insecticidal activity against larvae of plant insects and antimicrobial activity [38]. 2015;52:1239–57. Google Scholar. 2007;13:9–17. Hydrodynamics of burst swimming fish larvae ; a conceptual model approach. In this study, P. retrofractum synergized the toxicity of A. calamus at all doses. Summary of Invasiveness Top of page A. The LD10 and LD30 values were 3.294 and 6.735 µg/larva, respectively, for A. calamus and 1.448 and 3.213 µg/larva, respectively, for P. retrofractum. VB and WP wrote and reviewed the paper. Nursing protects honeybee larvae from secondary metabolites of pollen. Conversely, EST activity showed no significant difference between groups (p ≥ 0.05, df = 7) (Table 6). The glutathione-S-transferase method was modified from Oppenoorth et al. After 24 h, the surviving larvae were used for enzyme extraction to determine the activities of esterase and glutathione-S-transferase. Necessary cookies are absolutely essential for the website to function properly. Full Text Available Observasi pada larva kerapu kertang E. Males, on the other hand, klasifikaai more efficient at depositing lipid from ingested carbohydrates. 2013;33:815–26. All data are presented in Tables 1, 2, 3, 4, 5, 6. Each ethanolic crude extract was evaluated individually to determine efficacy levels upon topical application to the thorax region with various concentrations of extracts (2–140 µg/larva) using acetone as a carrier. HRMS (ESI) Calcd for C17H19NNaO3 308.1263 ([M+Na]+), Found 308.1278. The literature suggests that complex mixtures would be more effective than pure or only one crude extract [12, 13]. They are less toxic to nontarget organisms and mammals. J Vector Ecol. J Pharm Sci Innov. Induction of detoxification enzymes in phytophagous insects: role of insecticide synergists, larval age, and species. Shah P, Ghang M, Deshmukh P, Kulkarni Y, Joshi S, Vyas B, et al. Another Piperaceae plant in this research is P. nigrum, which is also among the top three for S. litura control. The mixtures containing 50 mM phosphate buffer (pH 7.2) were mixed with glutathione solution, supernatant, and 1-chloro-2,4′-dinitrobenzene (CDNB). The larva is klasifikasu in color based on age. Parasitol Res. Asian Pac J Trop Biomed. Acta Agric Zhejiangensis. h޴�mO�0ǿ�_n/�c'��e��H�/�ⵑҤJ��~>�)i��%L������s�����(B��D%��ّ������h �_�B�H�(�n��J��c|�h�c�m��Bx���cc}I�\봶p�-]do�.��8��E��-���p���xl}��_i^kk\ ���v_ rDN�6��\n���N�. Asian Pac J Trop Biomed. Hymenia recurvalis [Spoladea recurvalis], Spodoptera litura, Heliothis armigera and sometimes grasshoppers are the most harmful pests. Fan LS, Rita M, Dzolkhifli O, Mawardi R. Insecticidal properties of Piper nigrum fruit extracts and essential oils against Spodoptera litura. Among aqueous extracts of nine medicinal plants, P. retrofractum showed the highest level of activity against Cx. Dadang D, EvaDwi F, Djoko P. Effectiveness of two botanical insecticide formulations to two major cabbage insect pests on field application. Based on antifeedant activity, we hypothesized that this binary mixture affected detoxification enzyme activity.